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KMID : 0379120100380010001
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Korean Journal of Mycology
2010 Volume.38 No. 1 p.1 ~ p.4
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Characteristic of Orchid Mycorrhizal Fungi from Roots of Cypripedium japonicum and C. macranthum
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Sim Mi-Young
Youm Jae-Young
Chung Jae-Min
Lee Byung-Chun
Koo Chang-Duck
Eom Ahn-Heum
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Abstract
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Orchid mycorrhizal fungi(OMF) were examined in roots of the two threatened orchid species, Cypripedium japonicum and C. macranthum. The morphological characteristics of mycorrhizal colonization in the roots of two orchid species were observed. OMF colonized in the roots of two species were identified using molecular analysis. DNA from the root was extracted and amplified internal transcribed spacer(ITS) region using basidiomycete ITS primers, ITS1-OF and ITS4-OF. Four species belonging to Tulasnellaceae in roots of C. japonicum and two species of Tulasnellaceae and one basidiomycetous species was found in roots of C. macranthum.
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KEYWORD
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Cypripedium japonicum, Cypripedium macranthum, OMF, Orchid mycorrhizal fungi, Peloton, Tulasnellaceae
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